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Changes in the behavioral and immunological parameters of the mollusk Biomphalaria tenagophila induced by disruption of the circadian cycle as a consequence of continuous illumination BJMBR
Waissel,I.; Mineo,J.R.; Natal,C.L..
In the present investigation we studied some behavioral and immunological parameters of adult gastropod mollusk, Biomphalaria tenagophila, which have been reproducing for several generations under laboratory conditions. One group of gastropods was kept on a 14-h light/10-h dark cycle, corresponding to a regular circadian cycle, and another group was exposed to continuous light for 48 h. Animals were studied along (behavioral groups) or immediately after (immunological groups) 48 h of regular circadian cycle or continuous light conditions. Stopping/floating, dragging and sliding were the behavioral aspects considered (N = 20 for regular cycle; N = 20 for continuous illumination) and number of hemocytes/µl hemolymph was the immunological parameter studied (N...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Circadian cycle; Mollusk; Neuroimmunology; Stress; Behavior.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999001200014
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Immune response to respiratory syncytial virus in young Brazilian children BJMBR
Queiróz,D.A.O.; Durigon,E.L.; Botosso,V.F.; Ejzemberg,B.; Vieira,S.E.; Mineo,J.R.; Yamashita,C.; Hein,N.; Lopes,C.L.; Cacharo,A.L.; Stewien,K.E..
We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Respiratory syncytial virus; Peripheral blood mononuclear cells; Young children; Surface antigens; Cytokines.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011
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Myosin V and iNOS expression is enhanced in J774 murine macrophages treated with IFN-gamma BJMBR
Reis,D.S.; Souza,M.A.; Mineo,J.R.; Espindola,F.S..
Actin-based motor protein requirements and nitric oxide (NO) production are important features of macrophage activity during phagocytosis or microbicidal processes. Different classes of myosins contribute directly or indirectly to phagocytosis by providing mechanical force for phagosome closure or organelle movement. Recent data have shown the presence of myosins IC, II, V and IXb in phagosomes of bone marrow-derived murine macrophages. In our investigation we demonstrated the presence of different classes of myosins in J774 macrophages. We also analyzed the effect of gamma interferon (IFN-gamma), with or without calcium ionophore or cytochalasin B, on myosins as well as on inducible nitric oxide synthase (iNOS) expression and NO production. Myosins IC,...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Myosins; Macrophages; IFN-gamma; INOS.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000200009
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Toxoplasma gondii and Neospora caninum serological status of different canine populations from Uberlândia, Minas Gerais Arq. Bras. Med. Vet. Zootec.
Mineo,T.W.P.; Silva,D.A.O.; Näslund,K.; Björkman,C.; Uggla,A.; Mineo,J.R..
Este trabalho teve como objetivo analisar a ocorrência de anticorpos contra Toxoplasma gondii e Neospora caninum em cães da cidade de Uberlândia, MG. As taxas de reatividade sorológica dos animais frente a esses parasitos foram comparadas em relação à sua origem e condições de vida. Um total de 369 amostras de soro canino foi obtido de três grupos de cães: (A) 213 pacientes do Hospital Veterinário da Universidade Federal de Uberlândia, (B) 62 pacientes de clínicas veterinárias particulares da cidade e (C) 94 cães errantes da cidade. As amostras foram analisadas para a presença de anticorpos contra os parasitos por meio de ELISA. Os resultados mostraram soropositividades de 30,3% para T. gondii, 9,2% para N. caninum e 5,7% para ambos os parasitos. O grupo...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Dog; Serum profile; Toxoplasma gondii; Neospora caninum.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000300022
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